The Characterization and Activity of Protease Enzyme on Different Days of Tadpoles [Pelophylax ridibundus (Pallas, 1771)]
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CitationSereflisan, H., Alkaya, A., Gokcek, K. (2019). The characterization and activity of protease enzyme on different days of tadpoles [Pelophylax ridibundus (Pallas, 1771)]. Aquatic Sciences and Engineering, 34(4), 112-115. https://doi.org/10.26650/ASE2019567360
In this study, protease enzyme activity of Pelophylax ridibundus (Pallas, 1771) tadpoles was determined during the larval period starting from 25 Gosner stage (1960). When the P ridibundus tadpoles reached the 25 Gosner stage (1960) on the 22nd day, the sampling was continued until the metamorphosis was completed by sampling at 5-day intervals. Initial average weight and length of the tadpoles were 0.076 g and 6.42 mm, respectively. The tadpoles were fed with pellet feed the size of 2 mm and 39% crude protein content twice a day in the morning and afternoon. The differences in the protease activity between the 22nd day and the 62nd day of the metamorphosis were found statistically significant (P<0.05). It has been observed that the protease enzyme reached maximum level at the 32nd day. In the next stages, protease activity showed a tendency to increase and decrease up to metamorphosis. These results can be used as the basis for the most appropriate nutritional formulation for P. ridibundus in the larval stage.