dc.contributor.author | Bereketoğlu, Ceyhun | |
dc.contributor.author | Nacar, Gözde | |
dc.contributor.author | Sarı, Tuğba | |
dc.contributor.author | Mertoğlu, Bülent | |
dc.contributor.author | Pradhan, Ajay | |
dc.date.accessioned | 2021-06-14T09:18:17Z | |
dc.date.available | 2021-06-14T09:18:17Z | |
dc.date.issued | 2021 | en_US |
dc.identifier.citation | Bereketoglu, C., Nacar, G., Sari, T., Mertoglu, B., & Pradhan, A. (2021). Transcriptomic analysis of nonylphenol effect on Saccharomyces cerevisiae. PeerJ, 9, e10794.
https://doi.org/10.7717/peerj.10794 | en_US |
dc.identifier.uri | https://doi.org/10.7717/peerj.10794 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12508/1764 | |
dc.description.abstract | Nonylphenol (NP) is a bioaccumulative environmental estrogen that is widely used as a nonionic surfactant. We have previously examined short-term effects of NP on yeast cells using microarray technology. In the present study, we investigated the adaptive response of Saccharomyces cerevisiae BY4742 cells to NP exposure by analyzing genome-wide transcriptional profiles using RNA-sequencing. We used 2 mg/L NP concentration for 40 days of exposure. Gene expression analysis showed that a total of 948 genes were differentially expressed. Of these, 834 genes were downregulated, while 114 genes were significantly upregulated. GO enrichment analysis revealed that 369 GO terms were significantly affected by NP exposure. Further analysis showed that many of the differentially expressed genes were associated with oxidative phosphorylation, iron and copper acquisition, autophagy, pleiotropic drug resistance and cell cycle progression related processes such as DNA and mismatch repair, chromosome segregation, spindle checkpoint activity, and kinetochore organization. Overall, these results provide considerable information and a comprehensive understanding of the adaptive response to NP exposure at the gene expression level. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | PeerJ Inc. | en_US |
dc.relation.isversionof | 10.7717/peerj.10794 | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Nonylphenol | en_US |
dc.subject | RNA-seq | en_US |
dc.subject | Saccharomyces cerevisiae | en_US |
dc.subject | OXPHOS | en_US |
dc.subject | Cell cycle process | en_US |
dc.subject.classification | Multidisciplinary Sciences | |
dc.subject.classification | Nonylphenols | |
dc.subject.classification | Terics | |
dc.subject.classification | Endocrine Disruptors | |
dc.subject.other | DNA-damage | |
dc.subject.other | Gene-expression | |
dc.subject.other | Budding yeast | |
dc.subject.other | Bisphenol-a | |
dc.subject.other | Toxicity | |
dc.subject.other | Proteins | |
dc.subject.other | Stress | |
dc.subject.other | Copper | |
dc.subject.other | Model | |
dc.subject.other | Biodegradation | |
dc.subject.other | Iron | |
dc.subject.other | Nonylphenol | |
dc.subject.other | Superoxide dismutase | |
dc.subject.other | Autophagy (cellular) | |
dc.subject.other | Bacterial growth | |
dc.subject.other | Bioaccumulation | |
dc.subject.other | Cell cycle progression | |
dc.subject.other | Centromere | |
dc.subject.other | Chromosome segregation | |
dc.subject.other | Controlled study | |
dc.subject.other | Down regulation | |
dc.subject.other | Gene cluster | |
dc.subject.other | Gene expression | |
dc.subject.other | Gene expression level | |
dc.subject.other | Gene ontology | |
dc.subject.other | Genetic transcription | |
dc.subject.other | Genome-wide association study | |
dc.subject.other | Gluconeogenesis | |
dc.subject.other | Kinetochore | |
dc.subject.other | M phase cell cycle checkpoint | |
dc.subject.other | Microarray analysis | |
dc.subject.other | Mismatch repair | |
dc.subject.other | Mortality rate | |
dc.subject.other | Nonhuman | |
dc.subject.other | Oxidative phosphorylation | |
dc.subject.other | Pleiotropy | |
dc.subject.other | RNA isolation | |
dc.subject.other | RNA sequence | |
dc.subject.other | RNA sequencing | |
dc.subject.other | Saccharomyces cerevisiae | |
dc.subject.other | Transcriptomics | |
dc.subject.other | Upregulation | |
dc.title | Transcriptomic analysis of nonylphenol effect on Saccharomyces cerevisiae | en_US |
dc.type | article | en_US |
dc.relation.journal | PeerJ | en_US |
dc.contributor.department | Mühendislik ve Doğa Bilimleri Fakültesi -- Biyomedikal Mühendisliği Bölümü | en_US |
dc.identifier.volume | 9 | en_US |
dc.relation.tubitak | 118Y311 | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.contributor.isteauthor | Bereketoğlu, Ceyhun | |
dc.relation.index | Web of Science - Scopus - PubMed | en_US |
dc.relation.index | Web of Science Core Collection - Science Citation Index Expanded | |