dc.contributor.author | Başusta, Nuri | |
dc.contributor.author | Demirhan, Sefa Ayhan | |
dc.contributor.author | Çiçek, Erdoğan | |
dc.contributor.author | Başusta, Asiye | |
dc.date.accessioned | 12.07.201910:50:10 | |
dc.date.accessioned | 2019-07-12T22:07:01Z | |
dc.date.available | 12.07.201910:50:10 | |
dc.date.available | 2019-07-12T22:07:01Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Basusta, N., Demirhan, S. A., Cicek, E., Basusta, A. (2017). Comparison of staining techniques for age determination of some chondrichthyan species. Turkish Journal of Fisheries and Aquatic Sciences, 17(1), 41-49. doi: 10.4194/1303-2712-v17_1_06 | en_US |
dc.identifier.issn | 1303-2712 | |
dc.identifier.uri | https://doi.org/10.4194/1303-2712-v17_1_06 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12508/828 | |
dc.description | WOS: 000396367400006 | en_US |
dc.description | Science Citation Index Expanded | en_US |
dc.description.abstract | In this study, six chondrichthyan species (Raja clavata, Raja miraletus, Raja asterias, Torpedo marmorata, Rhinobatos rhinobatos and Gymnura altavela) were captured for age determination in Iskenderun Bay (North eastern Mediterranean Sea) between May 2010 and January 2012. Age was determined from vertebral sectioned, using four staining techniques, namely Crystal Violet, Silver Nitrate, Safranin O and Alcian Blue. The results from these four techniques were compared to each other, with respect to visibility success on growth rings. Of all four staining techniques, Silver Nitrate staining provided the best optical resolution for growth rings of R. clavata, R. miraletus and R. rhinobatos. For, R. asterias, T. marmorata and G. altavela, however, it provided a lower optical resolution. In fact, four staining technique was also efficient for R. clavata. The most efficient staining techniques were Safranin O in T. marmorata and Alcian Blue in R. rhinobatos. The order of average readability scores for staining techniques was as follows: Silver Nitrate = Safranin O > Crystal Violet = Alcian Blue. A standardized staining methodology that can be used for all species studied, however, has not been achieved. | en_US |
dc.description.sponsorship | Scientific and Technological Research Council of Turkey (TUBITAK) [TOVAG 109O634] | en_US |
dc.description.sponsorship | The authors thank E. I. Ozer, H. Girgin, O. V. Duman, for preparation, sectioning, and staining of centra. An experimental fishing permit was granted to us by the General Directorate of Fisheries and Aquaculture, Ministry of Food, Agriculture and Livestock of the Turkish Republic to collect elasmobranchs in these locations during the months of April, May and June, when these waters are closed to commercial fishing. Funding was provided by the Scientific and Technological Research Council of Turkey (TUBITAK), Project No: TOVAG 109O634. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Central Fisheries Research Inst | en_US |
dc.relation.isversionof | 10.4194/1303-2712-v17_1_06 | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Age Determination | en_US |
dc.subject | Cartilaginous Fish | en_US |
dc.subject | Crystal Violet | en_US |
dc.subject | Silver Nitrate | en_US |
dc.subject | Alcian Blue | en_US |
dc.subject | Safranin O | en_US |
dc.subject.classification | Shark | Sharks | Shark populations | en_US |
dc.subject.classification | Fisheries | Marine & Freshwater Biology | en_US |
dc.subject.other | dogfish squalus-acanthias | en_US |
dc.subject.other | iskenderun bay | en_US |
dc.subject.other | school shark | en_US |
dc.subject.other | growth | precision sea |
dc.subject.other | precision | en_US |
dc.subject.other | sea | en_US |
dc.title | Comparison of staining techniques for age determination of some chondrichthyan species | en_US |
dc.type | article | en_US |
dc.relation.journal | Turkish Journal of Fisheries and Aquatic Sciences | en_US |
dc.contributor.department | Deniz Bilimleri ve Teknolojisi Fakültesi | en_US |
dc.contributor.authorID | 0000-0002-4260-4772 | en_US |
dc.identifier.volume | 17 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.startpage | 41 | en_US |
dc.identifier.endpage | 49 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.contributor.isteauthor | Demirhan, Sefa Ayhan | |
dc.relation.index | Web of Science (ESCI) - Scopus - TR-Dizin | en_US |