dc.contributor.author | Yola, Mehmet Lütfi | |
dc.contributor.author | Atar, Necip | |
dc.contributor.author | Özcan, Nermin | |
dc.date.accessioned | 2021-06-11T12:23:54Z | |
dc.date.available | 2021-06-11T12:23:54Z | |
dc.date.issued | 2021 | en_US |
dc.identifier.citation | Yola, M. L., Atar, N., & Özcan, N. (2021). A novel electrochemical lung cancer biomarker cytokeratin 19 fragment antigen 21-1 immunosensor based on Si3N4/MoS2 incorporated MWCNTs and core-shell type magnetic nanoparticles. Nanoscale, 13(8), 4660–4669.
https://doi.org/10.1039/d1nr00244a | en_US |
dc.identifier.uri | https://doi.org/10.1039/d1nr00244a | |
dc.identifier.uri | https://hdl.handle.net/20.500.12508/1759 | |
dc.description.abstract | Lung cancer is one of deadliest and most life threatening cancer types. Cytokeratin 19 fragment antigen 21-1 (CYFRA 21-1) is a significant biomarker for the diagnosis of non-small cell lung cancer (NSCLC). Due to these reasons, a novel electrochemical immunosensor based on a silicon nitride (Si3N4)-molybdenum disulfide (MoS2) composite on multi-walled carbon nanotubes (Si3N4/MoS2-MWCNTs) as an electrochemical sensor platform and core-shell type magnetic mesoporous silica nanoparticles@gold nanoparticles (MMSNs@AuNPs) as a signal amplifier was presented for CYFRA21-1 detection in this study. Capture antibody (Ab(1)) immobilization on a Si3N4/MoS2-MWCNT modified glassy carbon electrode (Si3N4/MoS2-MWCNTs/GCE) was firstly successfully performed by stable electrostatic/ionic interactions between the -NH2 groups of the capture antibody and the polar groups of Si3N4/MoS2. Then, specific antibody-antigen interactions between the electrochemical sensor platform and the signal amplifier formed a novel voltammetric CYFRA21-1 immunosensor. The prepared composite materials and electrochemical sensor surfaces were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). A linearity range of 0.01-1.0 pg mL(-1) and a low detection limit (LOD) of 2.00 fg mL(-1) were also obtained for analytical applications. Thus, the proposed immunosensor based on Si3N4/MoS2-MWCNTs and MMSNs@AuNPs has great potential for medical diagnosis of lung cancer. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Royal Society of Chemistry | en_US |
dc.relation.isversionof | 10.1039/d1nr00244a | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject.classification | Immunosensors | |
dc.subject.classification | Carcinoembryonic antigen | |
dc.subject.classification | Alpha fetoprotein | |
dc.subject.classification | Chemistry | |
dc.subject.classification | Multidisciplinary | |
dc.subject.classification | Nanoscience & Nanotechnology | |
dc.subject.classification | Materials Science | |
dc.subject.classification | Multidisciplinary | |
dc.subject.classification | Physics | |
dc.subject.classification | Applied | |
dc.subject.other | Antigens | |
dc.subject.other | Neoplasm | |
dc.subject.other | Biomarkers | |
dc.subject.other | Tumor | |
dc.subject.other | Biosensing techniques | |
dc.subject.other | Carcinoma | |
dc.subject.other | Non-Small-Cell lung | |
dc.subject.other | Electrochemical techniques | |
dc.subject.other | Electrodes | |
dc.subject.other | Gold | |
dc.subject.other | Humans | |
dc.subject.other | Immunoassay | |
dc.subject.other | Keratin-19 | |
dc.subject.other | Limit of detection | |
dc.subject.other | Lung neoplasms | |
dc.subject.other | Magnetite nanoparticles | |
dc.subject.other | Metal nanoparticles | |
dc.subject.other | Molybdenum | |
dc.subject.other | Nanotubes | |
dc.subject.other | Carbon | |
dc.subject.other | Antibodies | |
dc.subject.other | Antigen-antibody reactions | |
dc.subject.other | Antigens | |
dc.subject.other | Biological organs | |
dc.subject.other | Biomarkers | |
dc.subject.other | Cyclic voltammetry | |
dc.subject.other | Diagnosis | |
dc.subject.other | Diseases | |
dc.subject.other | Drug products | |
dc.subject.other | Electrochemical impedance spectroscopy | |
dc.subject.other | Fourier transform infrared spectroscopy | |
dc.subject.other | Glass membrane electrodes | |
dc.subject.other | Gold nanoparticles | |
dc.subject.other | High resolution transmission electron microscopy | |
dc.subject.other | Immunosensors | |
dc.subject.other | Keratin | |
dc.subject.other | Layered semiconductors | |
dc.subject.other | Magnetic nanoparticles | |
dc.subject.other | Molybdenum compounds | |
dc.subject.other | Multiwalled carbon nanotubes (MWCN) | |
dc.subject.other | Scanning electron microscopy | |
dc.subject.other | Shells (structures) | |
dc.subject.other | Silica | |
dc.subject.other | Silica nanoparticles | |
dc.subject.other | Silicon nitride | |
dc.subject.other | Sulfur compounds | |
dc.subject.other | X ray photoelectron spectroscopy | |
dc.subject.other | Analytical applications | |
dc.subject.other | Diagnosis of lung cancer | |
dc.subject.other | Electrochemical immunosensors | |
dc.subject.other | Magnetic mesoporous silicas | |
dc.subject.other | Modified glassy carbon electrode | |
dc.subject.other | Molybdenum disulfide | |
dc.subject.other | Non small cell lung cancer | |
dc.subject.other | Specific antibody-antigen interaction | |
dc.subject.other | Electrochemical sensors | |
dc.subject.other | Antigen CYFRA21.1 | |
dc.subject.other | Cytokeratin 19 | |
dc.title | A novel electrochemical lung cancer biomarker cytokeratin 19 fragment antigen 21-1 immunosensor based on Si3N4/MoS2incorporated MWCNTs and core-shell type magnetic nanoparticles | en_US |
dc.type | article | en_US |
dc.relation.journal | Nanoscale | en_US |
dc.contributor.department | Mühendislik ve Doğa Bilimleri Fakültesi -- Biyomedikal Mühendisliği Bölümü | en_US |
dc.identifier.volume | 13 | en_US |
dc.identifier.issue | 8 | en_US |
dc.identifier.startpage | 4660 | en_US |
dc.identifier.endpage | 4669 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.contributor.isteauthor | Özcan, Nermin | |
dc.relation.index | Web of Science - Scopus - PubMed | en_US |
dc.relation.index | Web of Science Core Collection - Science Citation Index Expanded | |