Electrochemical Tau Protein Immunosensor Based on MnS/GO/PANI and Magnetite-incorporated Gold Nanoparticles
AuthorYola, Bahar Bankoğlu
Yola, Mehmet Lütfi
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CitationYola, B.B., Karaman, C., Özcan, N., Atar, N., Polat, İ., Yola, M.L. (2022). Electrochemical Tau Protein Immunosensor Based on MnS/GO/PANI and Magnetite-incorporated Gold Nanoparticles. Electroanalysis, 34 (9), pp. 1519-1528. https://doi.org/10.1002/elan.202200159
Herein, electrochemical tau protein immunosensor based on manganese sulfide nanoparticles/graphene oxide/polyaniline (MnS/GO/PANI) and magnetite-incorporated gold nanoparticles (AuNPs@Fe3O4) was constructed. After the modification of the glassy carbon electrode (GCE) with MnS/GO/PANI, the immobilization and conjugation of anti-Tau capture antibody and antigen Tau protein were successfully completed on MnS/GO/PANI/GCE, respectively. Then, the conjugation of anti-Tau secondary antibody was carried out to AuNPs@Fe3O4 as signal amplification via amino-gold affinity and the eventual immunosensor was accomplished by the distinctive interactions of electrode platform and signal amplification. Final immunosensor demonstrated the quantification limit (LOQ) of 1.0×10−13 M and the detection limit (LOD) of 1.0×10−14 M.